Post-doctoral Research in High Resolution Spatiotemporal Imaging of Mitotic Processes.

A Postdoctoral position is available at the Samuel Lunenfeld Institute Research Institute in Mount Sinai Hospital in Toronto. The Pelletier lab is keenly interested in the molecular mechanisms that govern various aspects of mitotic cell division including centrosome duplication, pericentriolar material assembly, microtubule dynamics, mitotic spindle formation and kinetochore function. Our studies revolve around cutting-edge quantitative microscopy approaches including structured-illumination, FRAP/FLIM, 4-D multiplex time-lapse imaging and high-content screening approaches in mammalian cells. Our imaging lab is equipped with 6 DeltaVision Elite deconvolution systems tweaked for fully automated high-resolution imaging of both live and fixed cells. Our laboratory also houses a 3D structured-illumination microscope allowing the study of the above-mentioned processes beyond the diffraction limit.

In this position, the successful applicant will be part of a fast-paced dynamic and multi-disciplinary environment and play an integral role in novel and ongoing projects within the laboratory. Applicants with experience in any of the following disciplines are preferred: advanced light microscopy, functional genomics and statistical approaches to quantitative microscopy and image analysis. Outstanding applicants in the field of molecular and cellular biology will also be considered.

Selected Publications:

Lawo S., Hasegan M., Gupta G.D. and Pelletier, L. Subdiffraction imaging of centrosomes reveals higher-order organizational features of pericentriolar material. Nature Cell Biology, October 21 (2012). PMID: 23086237

Lawo S, Bashkurov M, Mullin M, Ferreria MG, Kittler R, Habermann B, Tagliaferro A, Poser I, Hutchins JR, Hegemann B, Pinchev D, Buchholz F, Peters JM, Hyman AA, Gingras AC, Pelletier L. HAUS, the 8-Subunit Human Augmin Complex, Regulates Centrosome and Spindle Integrity. Current Biology, May 26;19(10):816-26 (2009). PMID: 19427217

Zhu, F.*, Lawo, S.*, Bird, A., Pinchev, D., Ralph, A., Richter, C., Müller-Reicher, T., Kittler, R., Hyman, A. and Pelletier, L. Cep192 regulates centrosome biogenesis in mammalian cells. Current Biology, 18(2):136-41) (2008). *Joint-first authors. PMID: 18207742
Kittler, R.*, Pelletier, L*, Heninger, A., Slabicki, M., Theis, M., Miroslaw, L., Poser, I., Lawo, S., Grabner, S., Kozak, K., Wagner, J., Surendranath, V., Richter, C., Bowen, W., Jackson, A.L., Habermann, B., Hyman, A.A. and Frank Buchholz. Genome-wide RNAi profiling of cell cycle progression in human tissue culture cells. Nature Cell Biology, 9(12):1401-1412 (2007). *Joint-first authors. PMID: 17994010

Pelletier, L., O'Toole, E.T., Schwager, A., Hyman, A.A. and Müller-Reichert, Centriole assembly in Caenorhabditis elegans. Nature, 444 (7119):619-23 (2006). PMID: 17136092

For more information visit:

http://www.mshri.on.ca/pelletier/