ポスドク募集
Duke University Medical Center, Department of Biochemistry
Yokoyama lab (http://www.biochem.duke.edu/modules/biochem_yokoyama_lab/index.php?id=1)

研究内容 ：
我々は生体内でのラジカル反応および金属の代謝に興味を持ち、下記のプロジェクトを進めております。
１．病原性細菌におけるエネルギーおよび核酸の代謝に関わるラジカル酵素の機構と制御。
２．様々な感染症の治療に用いられている酸化還元センサー抗生物質の作用機序。
３．モリブデン補酵素生合成酵素の反応機構解析と精神性疾患の関係。
参考文献（総説）：Schwarz, G., Cellular and Molecular Life Sciences 2005, 62, 2792-2810; Nordlund, P. and Reichard, R., Annu. Rev. Biochem. 2006, 75, 681-706.　過去の研究はWebサイトをご参照ください。

方法論：前定常状態酵素反応速度論解析、各種分光法（EPR, NMR, UV-vis, 蛍光）、非天然型アミノ酸の部位特異的導入（Schultz法）、タンパク質の蛍光標識、基質やリガンドを模倣した低分子プローブなど。
いずれのプロジェクトにおいても精製酵素の精密な反応機構解析とともに、細菌・細胞培養を用いた研究も行うことで生体内で起きている反応をより正確に理解することを目指しています。また、研究対象は特異なラジカル反応を触媒する酵素や金属イオンの代謝制御などに関わる酵素など、学術的に注目されている分野です。さらにこれらは疾患の原因、抗生物質の標的、もしくは抗生物質そのものであるために、現実社会の問題とも深くつながっています。このような対象を研究することで、より視野の広い研究を目指しています。

応募資格: 博士号取得者あるいは取得見込みのある方。
待遇：給与はNIHとDuke大学の規定によります。
着任時期：相談に応じます。
提出書類：次の書類をＥメールでお送り下さい。（１）興味を持った理由と過去の研究概要、（２）履歴書、（３）研究業績一覧、（４）推薦者3名以上の氏名と連絡先。
応募締切：適任者が決まり次第、募集を終了します。
書類送付先、問合せ先：横山健一 (ken.yoko@duke.edu, http://www.biochem.duke.edu/modules/biochem_yokoyama_lab/index.php?id=1)　日本語可。

POSTDOCTORAL POSITION

Mechanistic enzymology, radicals and metals in biology.

Kenichi Yokoyama Lab; Duke University Medical Center (http://www.biochem.duke.edu/modules/biochem_yokoyama_lab/index.php?id=1)

A postdoctoral position (or a research scientist position) is available for either of the following projects in the Yokoyama lab at Duke University Medical Center:
1. Mechanism and regulation of radical enzymes in nucleotide and energy metabolisms in pathogenic bacteria with strong emphasis on ribonucleotide reductases and pyruvate formate lyase.
2. Modes of action of redox sensing antibiotics targeting anaerobic and chronic infections.
3. Mechanism of enzymes in the molybdenum cofactor biosynthetic pathway and their relations to neurologic disease.

The successful candidate will use variety of pre-steady state kinetic methods combined with biophysical detection methods and/or chemical biology tools, which include site-specific unnatural amino acid incorporation, protein labeling and EPR/NMR spectroscopies. The successful candidate will also experience extensive mechanistic thinking and discussions in the interdisciplinary fields of chemistry, enzymology, biophysics, neurology and bacteriology.

Applicants should have profound experience in general protein expression/purifications and enzyme activity assays. Solid background in basic biochemistry or chemistry is required. A candidate must be strong in communication, focused and motivated to succeed, and able to work as a team as well as independently. We are also looking for specialists in human tissue culture, in vivo imaging and metabolomics/proteomics. Synthetic chemical background would also be appreciated.

Salary is commensurate with qualifications and experience based on Duke University or NIH scale. The position will be initially available for one year, with the renewal (up to 4-5 years) based on the performance and funding availability. The successful candidate will be encouraged to apply for independent fellowships to gain independent programs for his/her future career path. Send a cover letter (1-2 pages) outlining your research interest and career goal, current curriculum vitae, and a name and e-mail address of three references to:

Kenichi Yokoyama, Ph.D.
Assistant professor
Department of Biochemistry, Duke University School of Medicine
233 Nanaline H. Duke, Durham, NC, 27710
Tel (Office): (919) 684-8848
Tel (Cell): (919) 317-2783