A postdoctoral position is available in the Eppley Institute for Research in Cancer and Allied Diseases at the University of Nebraska Medical Center (http://www.unmc.edu/Eppley/). The candidate will use X-ray crystallography and a variety of biophysical and biochemical methods to understand the structural aspects of signal transduction and transcription in eukaryotes. A Ph.D. with a strong background in biochemistry, including a documented extensive experience in cloning, expression in E-coli and/or insect cells, and purification of mammalian proteins is required. Omaha is among the top 10 best US cities for living (http://money.cnn.com/magazines/moneymag/bplive/2006/top100/bigcities.html). Interested candidates are invited to submit their application, including cover letter, detailed curriculum vitae, statement of research experience and interests as well as names and addresses of at least three references to: Tahir H. Tahirov, Ph.D. Professor of Structural Biology, Eppley Institute for Research in Cancer and Allied Disease, University of Nebraska Medical Center, Lied Transplant Center Room 10737A, 987696 Nebraska Medical Center, Omaha, NE 68198-7696. E-mail: ttahirov@unmc.edu

References

Tahirov T.H., Misaki S., Meyer T.E., Cusanovich M.A., Higuchi Y., Yasuoka N. Concerted movement of side chains in the heme vicinity observed on ligand binding in cytochrome c' from Rhodobacter capsulatus. Nature Struct. Biol. 3, 459-464 (1996).

Tahirov T.H., Inoue-Bungo T., Morii H., Fujikawa A., Sasaki M., Kimura K., Shiina M., Sato K., Kumasaka T., Yamamoto M., Ishii S., Ogata K. Structural analyses of DNA recognition by the AML1/Runx-1 Runt domain and its allosteric control by CBFβ. Cell 104, 755-767 (2001).

Tahirov TH, Sato K, Ichikawa-Iwata E, Sasaki M, Inoue-Bungo T, Shiina M, Takata S, Fujikawa A., Morii H, Kumasaka T, Yamamoto M, Ogata K. Mechanism of c-Myb–C/EBPβ cooperation from separated sites on a promoter. Cell 108, 57-70 (2002).

Tahirov TH, Temiakov D, Anikin M, Patlan V, McAllister WT, Vassylyev DG, Yokoyama S. Structure of T7 RNA polymerase elongation complex at 2.9 Å resolution. Nature 420, 43-50 (2002).

Perederina A, Svetlov V, Vassylyeva M, Artsimovitch I, Tahirov TH, Shigeyuki Y, Vassylyev DG. Regulation through the secondary structure – structural framework for pp-G-pp-DksA synergism during transcription. Cell 118, 297-309 (2004).

Chivers PT, Tahirov TH. Structure of Pyrocccus horikoshii NikR: nickel sensing and implications for the regulation of DNA recognition. J Mol Biol 348, 597-607 (2005).

Temiakov D, Zenkin N, Vassylyeva MN, Perederina A, Tahirov TH, Kashkina E, Savkina M, Zorov S, Nikiforov V, Igarashi N, Matsugaki N, Wakatsuki S, Severinov K, Vassylyev DG. Structural basis of transcription inhibition by antibiotic streptolydigin. Molecular Cell 19, 655-666 (2005).

Artsimovitch I, Vassylyeva MN, Svetlov D, Svetlov V, Perederina A, Igarashi N, Matsugaki N, Wakatsuki S, Tahirov TH, Vassylyev DG. Allosteric modulation of the RNA polymerase catalytic reaction is an essential component of transcription control by rifamycins. Cell 122, 351-363 (2005).

Inagaki E, Ohshima N, Takahashi H, Kuroishi C, Yokoyama S, Tahirov TH. Crystal Structure of Thermus thermophilus Δ1-Pyrroline-5-carboxylate Dehydrogenase. J Mol Biol 362, 490-501 (2006).

Vassylyev DG, Vassylyeva MN, Perederina A, Tahirov TH, Artsimovitch I. Structural basis for transcription elongation by bacterial RNA polymerase. Nature 448, 157-162 (2007).