Univ. of Alabama ポスドク募集のお知らせ

アラバマ大学のDr. Hengbin Wang がポスドクを募集しております。
興味がおありの方は Dr. Hengbin Wang に直接ご連絡してください。

Research interests:
The organization and function of chromatin, the physiological template for all nuclear processes involving genetic information, are largely dictated by posttranslational histone modifications. Our laboratory has been focused on studying one of these modifications, histone ubiquitination. By following the enzymatic activities, we have recently identified several enzymes that are responsible for ubiquitination and deubiquitination of histone H3, H4, H2A, H2B, and H1. Further characterization indicates that these enzymes play critical roles in gene expression, cell cycle control, and DNA damage repair (see publication list). We are currently investigating the roles of these enzymes in stem cell function, embryogenesis, and cancer development. We employ a combination of biochemical and genetic approach to decipher the functions of these histone modifying enzymes in chromatin and cellular regulation. People with strong Interests in chromatin and epigenetics are encouraged to apply.

Publication list:
1. Hengbin Wang and Yi Zhang (2001). Mi2, an autoantigen for dermatomyositis, is an ATP-dependent nucleosome remodeling factor. Nuclei Acids Research, 29,2517-2521.
2. Hengbin Wang, Zhiqing Huang, Li Xia, Qin Feng, Hediye Erdjument-Bromage, Brain D. Strahl, Scott Briggs, David C. Allis, Jiemin Wong, Paul Tempst, and Yi Zhang (2001). Methylation of histone H4 at arginine 3 facilitating transcriptional activation by nuclear hormone receptor. Science, 293, 853-857.
3. Hengbin Wang, Ru Cao, Li Xia, Hediye Erdjument-Bromage, Christoph Borchers, Paul Tempst, and Yi Zhang (2001). Purification and functional characterization of a histone H3-lysine 4-specific methyltransferase. Molecular Cell, 8,1207-1217.
4. Yang L., Xia L., Wu, D.Y., Wang H., Chansky, H.A. Schubach W.H., Hickstein D. D., Zhang Y. (2002). Molecular cloning of ESET, a novel histone H3-specific methyltransferase that interacts with ERG transcription factor. Oncogene 21, 148-152.
5. Huck −H Ng, Qin Feng, Hengbin Wang, Hediye Erdjument-Bromage, Paul Tempst, Yi Zhang, and Kevin Strahl (2002). Methylation of histone lysine 79 by Dot1 is required for telomere silencing in C. cerevisae. Genes and Development, 16, 1518-1527.
6. Jia Fang, Qin Feng, Carrie S. Ketel, Hengbin Wang, Ru Cao, Li Xia, Hediye Erdjument-Bromage, Paul Tempst, Jeffrey A. Simon, and Yi Zhang (2002). Purification and Characterization of SET8, a nucleosomal histone H4 lysine 20 specific methyltransferase. Current Biology, 9, 12(13),1086-99.
7. Qin Feng, Hengbin Wang, Huck-H Ng, Hediye Erdjument-Bromage, Paul Tempst, Kevin Strahl, and Yi Zhang (2002). Methylation of lysine 79 by Dot1L protein in mammalian cells. Current Biology, 25,12(12), 1052-1058.
8. Ru Cao, Liangjun Wang, Hengbin Wang, Li Xia, Hediye Erdjument-Bromage, Paul Tempst, Richard S. Jones, and Yi Zhang (2002). Role of Histone H3 lysine 27 methylation in polycomb-group silencing. Science, 298, 1039-1043.
9. Kathrin Plath, Jia Fang, Susanna K. Mlynarczyk-Evans, Ru Cao, Kathleen A Worringer, Hengbin Wang, Cecile C. de la Cruz, Arie P. Otte, Barbar Panning, and Yi Zhang (2003). Role of histone H3 lysine 27 methylation in X inactivation. Science, 300, 131-135.
10. Hengbin Wang, An Woojin, Ru Cao, Li Xia, Hediye Erdjument-Bromage, Bruno Chatton, Paul Tempst, Robert G. Roeder, and Yi Zhang (2003). mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression. Molecular Cell, 12 (2), 475-487.
11. Jia Fang, Hengbin Wang, and Yi Zhang (2004). Purification of histone methyltransferases from Hela cells. Methods in Enzymology, 377:213-226.
12. Hengbin Wang, Liangjun Wang, Migual Vadel, Hediye Erdjument-Bromage, Paul Tempst, Richard S. Hones, and Yi Zhang (2004). Role of H2A ubiquitination in Polycomb gene silencing. Nature 431:873-878.
13. Hengbin Wang*, Ling Zhai, Jun Xu, Heui-Yoo Joo, Sarah Jackson, Hediye Erdjument-Bromage, Paul Tempst, Yue Xiong, and Yi Zhang*. (2006). Histones H3 and H4 ubiquitylation by the Cul4-DDB-ROC1 ubiquitin ligase facilitates cellular response to DNA damage. Molecular Cell. 22(3):383-394. (*co-correspondence author).
14. Jianhua Wei, Ling Zhai, Jun Xu, and Hengbin Wang. (2006). Role of Bmi1 in H2A Ubiquitylation and Hox Gene Silencing, Journal of Biological Chemistry, Aug 11;281(32):22537-44.
15. Qian Dai and Hengbin Wang. (2006). Cullin 4 makes its mark on chromatin. Cell Div. Jul 10;1:14.
16. Heui-Yoo Joo, Ling Zhai, Chunying Yang, Shui Nie, Hediye Erdjument-Bromage, Paul Tempst, and Chenbei Chang, Hengbin Wang. Regulation of cell cycle progression and gene expression by H2A deubiquitination. (second Revision for Nature).
17. Ling Zhai, Jianhua Wei, and Hengbin Wang. Reconstitution and functional characterization of Cul4 E3 ligase complexes reveal a critical role of DDB2 in histone ubiquitylation. (in preparation).

Hengbin Wang, Ph.D.
Assistant Professor
Department of Biochemistry and Molecular Genetics
The University of Alabama at Birmingham
Kaul Human Genetics Building, 402A
720 South 20th Street
Birmingham, AL 35294-0024
Tel: 205-934-5286 (O); 205-934-5289 (L)
Fax: 205-934-0758
E-mail: hbwang@uab.edu